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KMID : 0368420040470040396
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Journal of Plant Biology
2004 Volume.47 No. 4 p.396 ~ p.400
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Effects of various pretreatments on seed germination ofCalystegia soldanella (Convolvulaceae), a coastal sand dune plant
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Ko Jung-Moon
Park Hye-Jeong
Min Byeong-Mee
Cha Hyeon-Cheol
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Abstract
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We tested various pretreatments to enhance the germination of ‘Sea bells’ (Calystegia soldanella Roem. et Schult). One experiment, which improved the germination rate by 70%, involved first scarifying the seed coats, then immersing the seeds in 50 ppm GA3 for 24 h. A GA3-alone pretreatment did not increase germination. However, the most effective method, with a 100% success rate, included 3 h of acid pretreatment with 98% H2SO4. We also used scanning electron microscopy (SEM) to examine the seed-coat surfaces and cross-sections of dry seeds. SEM showed structural differences between seeds that were not treated and those exposed to 98% H2SO4. The latter treatment allowed the seed coats to crack and break, thereby disrupting their physical dormancy.
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